Aldose reductase and advanced glycation end products formation inhibitory activity of standardized extracts of Picrorhiza kurroa (Royle ex benth) and Hibiscus rosa-sinensis (Linn.)

Anasuri Santhosh, C. Veeresham, A. Rama Rao

Abstract


Objective: The objectives were to study the in-vitro and in-vivo aldose reductase and in-vitro advanced glycation end products formation inhibitory activities of the standardized extracts of Picrorhiza kurroa roots and Hibiscus rosa-sinensis flowers.

Methods: In-vitro Aldose reductase inhibitory activity was studied by using isolated rat lens and kidney Aldose reductase by UV-Visible spectro photo metric method by using Quercetin as reference compound. In-vivo Aldose reductase inhibitory activity was evaluated by using experimental rat models of galactosemia and the final lens galactitol was evaluated by High performance liquid chromatography and Gas chromatography methods by using Quercetin as reference compound. In-vitro advanced glycation end products formation inhibitory activity was estimated by using laboratory test reaction with protein and sugars by spectro fluorimetric method by sung aminoguanidine as reference compound. Statistical analysis of the results was done by using Analysis of the variance method.

Results: The plant extracts were found to possess significant aldose reductase and advanced glycation end products formation inhibitory activity.

Conclusions: More study is required for isolation and characterization of the chief chemical constituents responsible for the biological activity of the plant extracts.


Keywords


Aldose reductase, Advanced glycation end products, Complications of diabetes mellitus, Picrorhiza kurroa, Hibiscus rosa-sinensis, Standardized extracts, Quercetin, Aminoguanidine

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DOI: http://dx.doi.org/10.26510/2394-0859.pbe.2017.28

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