Analytical method validation for estimation of avanafil and dapoxetine hydrochloride tablet dosage form by HPTLC method

Dhwani A. Shah, Kunjal L. Vegad, Ekta D. Patel, Hitesh K. Prajapati, Ronak N. Patel, Yogesh K. Patel

Abstract


Objective: A simple, specific, accurate and precise RP-HPTLC method has been developed and validated for simultaneous estimation of Avanafil and Dapoxetine.

Methods: The chromatographic separation was achieved on Aluminium plates precoated with Silica gel 60 F254 using chloroform: methanol: ethyl acetate: glacial acetic acid (5:2:3:0.2, v/v/v/v) as mobile phase detected at 279 nm.

Results: The correlation coefficient for RP-HPLC method was found to be 0.9987 for Avanafil and 0.9991 Dapoxetine and the linearity range was found to be 1040-3640 ng*spot-1 for Avanafil and 80-280 ng*spot-1 for Dapoxetine.

Conclusions: The developed method was successfully applied to marketed tablet dosage form and the results were found with higher confidence.

Keywords


Avanafil, Dapoxetine, RP-HPTLC, Validation

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References


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DOI: http://dx.doi.org/10.26510/2394-0859.pbe.2017.26

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