Analytical method validation for estimation of avanafil and dapoxetine hydrochloride tablet dosage form by HPTLC method

Dhwani A. Shah, Kunjal L. Vegad, Ekta D. Patel, Hitesh K. Prajapati, Ronak N. Patel, Yogesh K. Patel


Objective: A simple, specific, accurate and precise RP-HPTLC method has been developed and validated for simultaneous estimation of Avanafil and Dapoxetine.

Methods: The chromatographic separation was achieved on Aluminium plates precoated with Silica gel 60 F254 using chloroform: methanol: ethyl acetate: glacial acetic acid (5:2:3:0.2, v/v/v/v) as mobile phase detected at 279 nm.

Results: The correlation coefficient for RP-HPLC method was found to be 0.9987 for Avanafil and 0.9991 Dapoxetine and the linearity range was found to be 1040-3640 ng*spot-1 for Avanafil and 80-280 ng*spot-1 for Dapoxetine.

Conclusions: The developed method was successfully applied to marketed tablet dosage form and the results were found with higher confidence.


Avanafil, Dapoxetine, RP-HPTLC, Validation

Full Text:



Amin G, Chapla B, Pandya A, Dr.Kakadiya J, Baria D. Developement and Validation of Dual Wavelength UV Spectrophotometric Method for Simultaneous estimation of Tadalafil and Dapoxetine hydrochloride in their combined tablet dosage form. Int J Bio-sci. 2012;1(2):247-55.

Dhaduk D, Jadeja B, Patel H, Rathod B, Patel P. Estimation of Sildenafil citrate and Dapoxetine hydrochloride in their combined dosage form by HPLC method. Inv Rapid Pharma J. 2012;4:1-4.

Giri AD, Bhusali VK, Dhaneshwar SR. Validated HPLC method for Simultaneous quantitation of Tadalafil and Dapoxetine hydrochloride in bulk drug and formulation. Int J Pharma Sci. 2012;4(2):654-8.

Rajpara CS, Akhtar J, Khandhar A. Development and Validation of Stability Indicating RP-HPLC Method For Estimation of Tadalafil And Dapoxetine HCL In Solid Dosage Form, Pharmatutor Art-1387, Avanafil. Available at: Accessed on 4 April 2017.

Introduction of Dosage form. Available at: Accessed on 7 April 2017.



  • There are currently no refbacks.

Copyright (c) 2017 Pharmaceutical and Biological Evaluations

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

Creative Commons License


© Copyright 2018 - Pharmaceutical and Biological Evaluations