Green synthesis of silver nanoparticles by Aspergillus consortium and evaluating its anticancer activity against Human breast adenocarcinoma cell line (MCF7)

Rajeswari P., Samuel P., Vijayakumar J., Selvarathinam T., Sudarmani D.N.P., Amirtharaj K., Deenathayalan R.


Objective: The objective of the present study is to produce silver nanoparticles from marine fungi from south west coastal areas of Tamil Nadu and evaluating their potentials with special reference to anticancer activity.

Methods: Hand core pushing technique is adapted to collect marine sediment samples along the coastal environs. Distinguished Aspergillus colonies were isolated and identified by wet mount procedure. The characterized Aspergillus consortium was subjected to produce silver nanoparticles. The extracellularly synthesized nanoparticles were characterized. Silver nanoparticles were evaluated for anticancer activity against MCF7 cell line by MTT assay. The IC50 values were determined.

Results: Aspergillus consortium consist of A. niger, A. michelle and A. japonicus. Silver nanoparticles were extracellularly synthesized by the reduction of silver nitrate (AgNO3) to metallic silver (Ag+) ions results in the transformation of pale yellow to dark red colour. Constant shift was observed at 420 nm while monitoring the solution by UV-Vis spectrophotometer. The presence of important functional groups like –NH3 was confirmed by FT-IR Spectroscopy. Anticancer activity of the silver nanoparticles was evaluated against MCF7. There was 100% cell inhibition when concentration of the AgNPs reached 25 µg, 50 µg and 100 µg respectively in the test solution. Notably the IC50 value was found to be very lowest for the nanoparticles produced by A. japonicus and the value was found to be 1.47 µg/ml.

Conclusions: Aspergillus consortium was found to be an ideal mycobiosystem for the production of silver nanoparticles with potential anticancer activity.


Hand core pushing technique, wet mount, Aspergillus consortium, MCF7, MTT, UV-Vis spectrophotometer, FT-IR Spectroscopy, IC50

Full Text:



Nair LS, Laurencin CT. Silver nanoparticles: synthesis and therapeutic applications. J Biomed Nanotechnol. 2007;3:301–16.

Law N, Ansari S, Livens FR, Renshaw JC, Lloyd JR. The formation of nanoscale elemental silver particles via enzymatic reduction by Geobacter sulfurreducens. Appl Environ Microbiol. 2008;74:7090–3.

Franco-Molina AM, Mendoza-Gamboa E, Sierra-Rivera CA, Gomez-Flores RA, Zapata-Benavides P, Castillo-Tello P, et al. Antitumor activity of colloidal silver on MCF-7 human breast cancer cells. J Exp Clin Cancer Res. 2010;29:148–55.

Shankar SS, Rai A, Ankamwar B, Singh A, Ahmad A, Sastry M. Biological synthesis of triangular gold nanoprisms. Nat Mater. 2004;3:482–8.

Mohanpuria P, Rana NK, Yadav SK. Biosynthesis of nanoparticles: Tech-nological concepts and future applications. J Nanopart Res. 2008;10:507–17.

Narayanan KB, Sakthivel N. Biological synthesis of metal nanoparticles by microbes. Adv. Colloid Interface Sci. 2010;156:1–13.

Kelecom A. Secondary metabolites from marine microorganisms. Anais da Academia Brasileira de Ciencias (Annals of the Brazilian Academy of Sciences). 2002;74(1):151-70.

Konig GM, Wright AD. Marine natural products research: Current directions and future potential. Planta Medica. 1996;62:193-211.

Hwang IS, Lee J, Hwang JH, Kim KJ, Lee DG. Silver nanoparticles induce apoptotic cell death in Candida albicans through the increase of hydroxyl radicals. FEBS J. 2012;279:1327-38.

Warcup JH. The soil plate method for isolation of fungi from soil. Nature. 1950;166:117-8.

Astrid L. Preparation of Lactophenol Cotton Blue Slide Mounts. Community Eye Health. 1999;12(30):24.

Gilman JC. A Manual of Soil Fungi. 2nd Ed. Ames, Iowa: Iowa State College Press; 1971: 450.

Subramanian CV, Raghukumar S. Ecology of higher fungi in soils of marine and brackish environments in the around Madras. Veroff. Inst. Meeresforsch. Bremesh. 1974;5:377-402.

Raper KB, Thom C. A manual of Penicillia. Williams and Wilkins. Baltimore; 1949: 875.

Domsch KH, Gams W and Andersen TH. Compendium of soil fungi. Academic Press, New York, USA; 1980.

Basavaraja S, Balaji SD, Arunkumar L, Rajasab AH, Venkataraman A. Extracellular biosynthesis of silver nanoparticles using the fungus Fusarium semitectum. Materials Research Bulletin. 2008;43(5):1164–70.

Bhainsa KC, D'Souza SF. Extracellular biosynthesis of silver nanoparticle using the fungus Aspergillus fumigatus. Colloids Surf B, Biointerfaces. 2006;47:160-4.

Vigneshwaran N, Ashtaputre NM, Varadarajan PV, Nachane RP, Paralikar KM, Balasubramanya RH. Biological Synthesis of silver nanoparticles using the fungus Aspergillus flavus. J Mater Lett. 2007;67:1413-8.

Talebia S, Ramezanib F, Ramezani M. Biosynthesis of metal nanoparticles by microorganisms. Nonocon. 2010;10:112-8.

Christophersen C, Crescente O, Frisvad JC, Gram L, Nielsen J, Nielsen PH, et al. Antibacterial activity of marine-derived fungi. Mycopathologia. 1999;143:135–8.

Sponga F, Cavaletti L, Lazzarini A, Borghi A, Ciciliato I, Losi D, et al. Biodiversity and potentials of marine-derived micro-organisms. Journal of Biotechnology and Biotechnological Aspects of Marine Sponges. 1999;70:65-9.

Lingheswar S, Jenila RD. Evaluation Profile of Silver Nanoparticle Synthesized By Aloe Vera Extract. International Journal of Chem Tech Research. 2014;6(9):4379–85.

Valentin BB, Smriti G, Usha Nandhini S. Silver nanoparticles synthesized from marine fungi Aspergillus oryzae. International Journal of Chem Tech Research. 2014;7(1):68–72.

Saeed M, Behroz M, Reza AG, Mehdi Y. Biological synthesis of silver nanoparticles by Aspergillus flavus, isolated from soil of Ahar copper mine. Indian Journal of Science and Technology. 2012;5(S3):2443–4.

Khabat V, Ali MG, Sedighe K. Biosynthesis of Silver Nanoparticles by Fungus Trichoderma Reesei (A Route for Large-Scale Production of AgNPs). Insciences J. 2011;1(1):65-79.

Reena S, Sunil KS, Muthusamy T. Biosynthesis of Silver Nanoparticles by Marine Invertebrate (Polychaete) and Assessment of Its Efficacy against Human Pathogens. Journal of Nanoparticles. 2014; 1-7.

Revathy M, Mathiazhagan A, Annadurai G. Biosynthesis, characterization and antibacterial activity of silver nanoparticles using the lichen Parmotrema Perlatum. EJBPS. 2015;2(4):348–61.

Saraniya DJ, Valentin B, Krupa R. Invitro anticancer activity of silver nanoparticles synthesized using the extract of Gelidiella sp. Int J Pharm Pharm Sci. 2012;4(Suppl 4):710-5.

Chandran SP, Chaudhary M, Pasricha R, Ahmad A, Sastry M. Synthesis of Gold Nanotriangles and Silver Nanoparticles Using Aloevera Plant Extract. Biotechnol Prog. 2006;22:577.

Valentin BB, Saraniya DJ, Usha NS. Green synthesis of cytotoxicity of silver nanoparticles from the extracts of marine macroalgae Gracilaria coricata. Indian J Biotechnol. 2015;14:276-81.

Sangiliyandi G, Jae WH, Vasuki E, Muniyandi J, Jin HK. Cytotoxicity of Biologically Synthesized Silver Nanoparticles in MDA-MB-231 Human Breast Cancer Cells. Biomed Res Int. 2013;2013:535796.

Vidhyashree N, Yamini sudha lakshmi S. Screening, isolation, identification, characterisation and applications of silver nanoparticles synthesized from marine Actinomycetes (S. grieseorubens). WJPR. 2015;4(8):1801-20.



  • There are currently no refbacks.

Copyright (c) 2017 Pharmaceutical and Biological Evaluations

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

Creative Commons License


© Copyright 2018 - Pharmaceutical and Biological Evaluations