Screening and characterisation of bioplastics producing bacteria isolated from oil contaminated soils of Virudhunagar, Tamil Nadu, India

K. Susithra, U. Ramesh, M. Kannan, R. Ganesan, K. Rajarathinam


Objective: To screen the high yielding PHB producing bacterial strains and its characterization by phenotypic methods and PHB production was optimized by using different carbon sources, different levels of pH and temperature.

Methods: The oil contaminated soils of Virudhunagar town were chosen for the isolation of PHB producing bacterial species. The bacterial isolates were stained by Sudan Black B method for observing the presence of PHB granules and further confirmation by UV and FTIR analysis. The PHB accumulations in bacterial isolates were done by UV-Spectrophotometric analysis. The maximum PHB production was optimized by varying pH, temperature and carbon sources used in the production medium.

Results: From the microscopic observations of the individual isolates, seven bacterial isolates were seen by the PHB granules inside the cell. The UV results interpret maximum amount 1052 microgram/ml of PHB by the second bacterial isolate. FTIR confirmed C=O group in the extracted PHB from production media. Also high yield was observed in at pH 9, 370 C and starch.

Conclusions: These PHB have potential candidate for some application in packaging and biomedical material production in the form of drug carriers.


PHB production, Optimization, pH, Temperature, Carbon source, FT-IR

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Abe HY. Molecular and material design of biodegradable poly(hydroxyl-alkanate)s’, in Doi Y and Steinbuchel A, Biopolymers 3b,polysters II, Weinheim, Wiley –VCH; 2002: 105-132.

Braunegg G, Lefebvre G, Genser KF. Poly-hydroxyalkanoates, biopolysters from renewable resources: Physiological and engineering aspects. J Biotechnol. 1998;65:127-61.

Lee SY. Bacterial poydroxyalkanoates. Biotechnol/Bioeng. 1996;49:1-4.

Steinbuchel A, Valentin H. Diversity of bacterial polyhydroxyalkanoic acids. FEMS Microbiology letters. 1995;128:219-28.

Agus J, Abe H, Doi Y, Tsuge T. Molecular weight characterization of poly[(R)-3-hydroxy-butyrate] synthesized by genetically engineered strains of Escherchia coli. Polym Degrad stab. 2006a;91:1138-46.

Padermshoke A, Sato H, Katsumoto Y, Ekgasit S, Noda I, Ozaki Y. Thermally induced phase transition of Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) investigated by two-dimensional infrared correlation spectroscopy. VibSpectrosc. 2005;36:241-9.

Volova TG, Kozhevnikov IV, Dolgopolova Y, Trusova M, Kalacheva GS, Arefeva YV. Physiological and biochemical characteristics and capacity for polyhydroxyalkanoates synthesis in a glucose –utilizing strain of hydrogen-oxidizing bacteria. Mikrobiologiia. 2005;74(6):788-94.

Rawte T, Mavinkurve S. Polyhydroxybutyrates from mangrove flora. Annual conference of Association of Microbiologists of India, Manglore; 1998: 241.

Ramsay JA, Hassan MC, Ramsay BA, Hemicellulose as a potential substrate for production of (poly-ß-hydroxybutyrates). Canadian J Microbiol. 1995;41(13):262-6.

Senthilkumar B, Prabhakaran G. Production of PHB (bioplastics) using bio-effluents as substrates by Alcaligenes eutropha. Indian J Biotechnol. 2006;5:76-9.

Sujatha K, Mahalakshmi A, Shenbagarathi. A study on accumulation of PHB in native pseudomonas isolates LCD-5 and LCD-25. Indian J Biotechnol. 2005;4:216-221.

Patwarthan PR, Srivastava AK. Model-based fed-batch cultivation of R.eutropha for enhanced biopolymer production. Biochem Eng J. 2004;20:21-8.



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